Transcription-coupled repair in yeast is independent from ubiquitylation of RNA pol II: implications for Cockayne's syndrome.

@article{Lommel2000TranscriptioncoupledRI,
  title={Transcription-coupled repair in yeast is independent from ubiquitylation of RNA pol II: implications for Cockayne's syndrome.},
  author={Lori Lommel and Miriam E. Bucheli and Kevin S. Sweder},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2000},
  volume={97 16},
  pages={9088-92}
}
Cockayne's syndrome cells lack transcription-coupled nucleotide excision repair (TCR) and ubiquitylation of RNA polymerase II large subunit (RNA pol II LS), suggesting that ubiquitylation of RNA pol II LS may be necessary for TCR in eukaryotes. Rsp5 is the sole yeast ubiquitin-protein ligase that ubiquitylates RNA pol II LS in cells exposed to DNA-damaging agents. In yeast lacking functional Rsp5, there is no ubiquitylation of RNA pol II LS. We show here that removal, repression, or over… CONTINUE READING