Transcript quantification based on chemical labeling of RNA associated with fluorescent detection.

@article{Fontaine2001TranscriptQB,
  title={Transcript quantification based on chemical labeling of RNA associated with fluorescent detection.},
  author={Lisa Fontaine and Sergine Even and Phillipe Soucaille and Nic D. Lindley and Muriel Cocaign-Bousquet},
  journal={Analytical biochemistry},
  year={2001},
  volume={298 2},
  pages={246-52}
}
A general method for RNA measurement, based on chemical labeling of RNA with digoxigenin (without retrotranscription), has been established. Labeled RNA is hybridized with nylon membranes containing spot blots of PCR-amplified gene fragments and the fluorescence detection is mediated via specific anti-digoxigenin antibody coupled to alkaline phosphatase. The method was optimized in order to be quantitative, and high precision (less than 24% error) was obtained, allowing analysis of relatively… CONTINUE READING

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