Transcript profiling of functionally related groups of genes during conditional differentiation of a mammalian cochlear hair cell line.

@article{Rivolta2002TranscriptPO,
  title={Transcript profiling of functionally related groups of genes during conditional differentiation of a mammalian cochlear hair cell line.},
  author={Marcelo N. Rivolta and Antony Halsall and Claire M. Johnson and Michael A. Tones and Matthew C. Holley},
  journal={Genome research},
  year={2002},
  volume={12 7},
  pages={1091-9}
}
We have used Affymetrix high-density gene arrays to generate a temporal profile of gene expression during differentiation of UB/OC-1, a conditionally immortal cell line derived from the mouse cochlea. Gene expression was assessed daily for 14 days under differentiating conditions. The experiment was replicated in two separate populations of cells. Profiles for selected genes were correlated with those obtained by RT-PCR, TaqMan analysis, immunoblotting, and immunofluorescence. The results… CONTINUE READING

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