Transcript profiling and expression level mapping.

  title={Transcript profiling and expression level mapping.},
  author={Elena Potokina and Arnis Druka and Michael J. Kearsey},
  journal={Methods in molecular biology},
Transcript abundance data from cRNA hybridizations to Affymetrix microarrays can potentially be used to identify genetic markers to facilitate high-throughput genotyping. We have shown that it is easily possible to use the information from Affymetrix expression arrays to accurately identify over 4,000 robust polymorphic transcript-derived markers (TDMs). We developed the method to identity TDM polymorphisms from experiments involving two tissues in two commercial varieties of barley and their… CONTINUE READING