Transcript imaging of the development of human T helper cells using oligonucleotide arrays

  title={Transcript imaging of the development of human T helper cells using oligonucleotide arrays},
  author={Lars Rogge and Elisabetta Bianchi and Mauro Biffi and Elisa Bono and Sheng-Yung P. Chang and Heather C. Alexander and Christopher D. Santini and Giuliana Ferrari and Luigi Sinigaglia and M Seiler and Martin Neeb and Jan Mous and Francesco Sinigaglia and Ulrich Certa},
  journal={Nature Genetics},
Many pathological processes, including those causing allergies and autoimmune diseases, are associated with the presence of specialized subsets of T helper cells at the site of inflammation. Understanding the genetic program that controls the functional properties of T helper type 1 (Th1) versus T helper type 2 (Th2) cells may provide insight into the pathophysiology of inflammatory diseases. We compared the gene-expression profiles of human Th1 and Th2 cells using high-density oligonucleotide… 

Gene expression analysis of Th1 and Th2 cells: clues to homing in inflammation

This work compared the gene expression profiles of human Th1 and Th2 cells using high-density oligonucleotide arrays with the capacity to display transcript levels of 6000 human genes and identified more than 200 differentially expressed genes, including genes controlling the different steps of lymphocyte migration and homing1.

Distinct gene expression profiles of human type 1 and type 2 T helper cells

This study demonstrates the power of the targeted use of microarrays in combination with quantitative real-time RT-PCR in identifying and validating new marker genes for gene expression studies.

Identification of global regulators of T-helper cell lineage specification

The results presented here show the involvement of several new actors in the early differentiation of T-helper cells and will be a valuable resource for better understanding of autoimmune processes.

An insight into molecular mechanisms of human T helper cell differentiation

In this review, what is known about human T helper differentiation is summarized and selected differences between human and mouse will be discussed.

Proteome profiling of interleukin‐12 treated human T helper cells

Compared to previous reports, this analysis provides a new view on the IL‐12 induced changes on CD4+ T cells underscoring the importance of creating and combining the data generated at various levels to build a comprehensive view of a given biological process of the cell.

Proteome characterization of human T helper 1 and 2 cells

Two proteins were differentially modified in the two cell types, and N‐terminal acetylation of cyclophilin A was stronger in Th1 than in Th2 cells, which highlights the complementary roles that proteomics and transcriptomics have in the elucidation of biological phenomena.

Differentiation Involved in Early Th1 and Th2 Cell Genome-Wide Identification of Novel Genes

Genes coding for components of certain pathways, such as RAS oncogene family and G protein-coupled receptor signaling, are revealed to be differentially regulated during the early Th1 and Th2 cell differentiation.

Genome-Wide Identification of Novel Genes Involved in Early Th1 and Th2 Cell Differentiation1

Among the novel genes identified, there may be many factors that play a crucial role in the regulation of the differentiation process together with the previously known factors and are potential targets for developing therapeutics to modulate Th1 and Th2 responses.



Lymphokine production by human T cells in disease states.

  • S. Romagnani
  • Biology, Medicine
    Annual review of immunology
  • 1994
A large body of evidence suggests the existence of polarized human T cell responses, reminiscent of Th1 and Th2 subsets described for mouse T cells, which appear to be involved in organ specific autoimmunity, in contact dermatitis, and in some chronic inflammatory disorders of unknown etiology.

Divergent T-cell cytokine patterns in inflammatory arthritis.

The experiments favor the possibility of therapeutic intervention in inflammatory rheumatic disease by means of inhibitory cytokines, and it was demonstrated that interleukin 4 is made by CD4 cells.

Shift toward T lymphocytes with a T helper 1 cytokine-secretion profile in the joints of patients with rheumatoid arthritis.

A shift toward T cells with a Th1 cytokine profile was observed in the joints of patients with RA, and this finding might have implications for the development of new therapies for RA.

Upregulation of Integrin 6/β1 and Chemokine Receptor CCR1 by Interleukin-12 Promotes the Migration of Human Type 1 Helper T Cells

Findings suggest that the exquisite regulation of integrin 6/β1 and CCR1 may play an important role in tissue-specific localization of Th1 cells.

P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflamed tissues

It is shown that Th1 cells, but not Th2 cells, are able to bind to P- selectin and E-selectin, indicating that selective recruitment is an additional level of regulation for both effector function profile and character of a local immune response.

The role of Stat4 in species-specific regulation of Th cell development by type I IFNs.

It is shown that activation of Stat4, which is necessary for the differentiation of naive T cells into polarized Th1 cells, is not sufficient to induce phenotype reversal of human Th2 cells, and restimulation ofhuman Th2 lines and clones in the presence of IFN-alpha does not induce the production ofIFN-gamma.