Transcript imaging of the development of human T helper cells using oligonucleotide arrays

@article{Rogge2000TranscriptIO,
  title={Transcript imaging of the development of human T helper cells using oligonucleotide arrays},
  author={Lars Rogge and Elisabetta Bianchi and Mauro Biffi and Elisa Bono and Sheng-Yung P. Chang and Heather C. Alexander and Christopher D. Santini and Giuliana Ferrari and Luigi Sinigaglia and M Seiler and Martin Neeb and Jan Mous and Francesco Sinigaglia and Ulrich Certa},
  journal={Nature Genetics},
  year={2000},
  volume={25},
  pages={96-101}
}
Many pathological processes, including those causing allergies and autoimmune diseases, are associated with the presence of specialized subsets of T helper cells at the site of inflammation. Understanding the genetic program that controls the functional properties of T helper type 1 (Th1) versus T helper type 2 (Th2) cells may provide insight into the pathophysiology of inflammatory diseases. We compared the gene-expression profiles of human Th1 and Th2 cells using high-density oligonucleotide… 

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References

SHOWING 1-10 OF 34 REFERENCES

Lymphokine production by human T cells in disease states.

  • S. Romagnani
  • Biology, Medicine
    Annual review of immunology
  • 1994
TLDR
A large body of evidence suggests the existence of polarized human T cell responses, reminiscent of Th1 and Th2 subsets described for mouse T cells, which appear to be involved in organ specific autoimmunity, in contact dermatitis, and in some chronic inflammatory disorders of unknown etiology.

Divergent T-cell cytokine patterns in inflammatory arthritis.

TLDR
The experiments favor the possibility of therapeutic intervention in inflammatory rheumatic disease by means of inhibitory cytokines, and it was demonstrated that interleukin 4 is made by CD4 cells.

Shift toward T lymphocytes with a T helper 1 cytokine-secretion profile in the joints of patients with rheumatoid arthritis.

TLDR
A shift toward T cells with a Th1 cytokine profile was observed in the joints of patients with RA, and this finding might have implications for the development of new therapies for RA.

Upregulation of Integrin 6/β1 and Chemokine Receptor CCR1 by Interleukin-12 Promotes the Migration of Human Type 1 Helper T Cells

TLDR
Findings suggest that the exquisite regulation of integrin 6/β1 and CCR1 may play an important role in tissue-specific localization of Th1 cells.

P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflamed tissues

TLDR
It is shown that Th1 cells, but not Th2 cells, are able to bind to P- selectin and E-selectin, indicating that selective recruitment is an additional level of regulation for both effector function profile and character of a local immune response.

The role of Stat4 in species-specific regulation of Th cell development by type I IFNs.

TLDR
It is shown that activation of Stat4, which is necessary for the differentiation of naive T cells into polarized Th1 cells, is not sufficient to induce phenotype reversal of human Th2 cells, and restimulation ofhuman Th2 lines and clones in the presence of IFN-alpha does not induce the production ofIFN-gamma.