Transcript-Assisted Transcriptional Proofreading

@article{Zenkin2006TranscriptAssistedTP,
  title={Transcript-Assisted Transcriptional Proofreading},
  author={Nikolay Zenkin and Yulia Yuzenkova and Konstantin V. Severinov},
  journal={Science},
  year={2006},
  volume={313},
  pages={518 - 520}
}
Fidelity of template-dependent nucleic acid synthesis is the main determinant of stable heredity and error-free gene expression. The mechanism (or mechanisms) ensuring fidelity of transcription by DNA-dependent RNA polymerases (RNAPs) is not fully understood. Here, we show that the 3′ end–proximal nucleotide of the nascent transcript stimulates hydrolysis of the penultimate phosphodiester bond by providing active groups and coordination bonds to the RNAP active center. This stimulation is much… 

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...

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