Transcript-Assisted Transcriptional Proofreading

@article{Zenkin2006TranscriptAssistedTP,
  title={Transcript-Assisted Transcriptional Proofreading},
  author={N. Zenkin and Yulia Yuzenkova and K. Severinov},
  journal={Science},
  year={2006},
  volume={313},
  pages={518 - 520}
}
  • N. Zenkin, Yulia Yuzenkova, K. Severinov
  • Published 2006
  • Biology, Medicine
  • Science
  • Fidelity of template-dependent nucleic acid synthesis is the main determinant of stable heredity and error-free gene expression. The mechanism (or mechanisms) ensuring fidelity of transcription by DNA-dependent RNA polymerases (RNAPs) is not fully understood. Here, we show that the 3′ end–proximal nucleotide of the nascent transcript stimulates hydrolysis of the penultimate phosphodiester bond by providing active groups and coordination bonds to the RNAP active center. This stimulation is much… CONTINUE READING
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    References

    SHOWING 1-10 OF 23 REFERENCES
    Fidelity of RNA polymerase II transcription controlled by elongation factor TFIIS.
    • C. Jeon, K. Agarwal
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1996
    • 121
    • PDF
    Mechanism of DmS-II-mediated pause suppression by Drosophila RNA polymerase II.
    • H. Guo, D. Price
    • Biology, Medicine
    • The Journal of biological chemistry
    • 1993
    • 46