Transcellular transport of creatinine in renal tubular epithelial cell line LLC-PK1.

  title={Transcellular transport of creatinine in renal tubular epithelial cell line LLC-PK1.},
  author={Yumiko Urakami and Naoko Kimura and Masahiro Okuda and Satohiro Masuda and Toshiya Katsura and Ken-ichi Inui},
  journal={Drug metabolism and pharmacokinetics},
  volume={20 3},
BACKGROUND/AIM Creatinine is excreted into urine via tubular secretion in addition to glomerular filtration. In the present study, characteristics of the creatinine transport in renal epithelial cells were investigated. METHODS The transcellular transport and accumulation of [14C]creatinine and [14C]tetraethylammonium (TEA) were assessed using LLC-PK1 cell monolayers cultured on porous membrane filters. RESULTS [14C]Creatinine was transported directionally from the basolateral to apical… 

Figures from this paper

A role for the organic anion transporter OAT3 in renal creatinine secretion in mice.

Renal creatinine clearance was consistently greater than inulin clearance (as a measure of GFR) in wild-type mice but not in mice lacking OAT1 (Oat1-/-) and Oat3 (OAT3-/-), indicating that the former condition enhanced the quantitative contribution of OAT3 for renalCreatinine secretion.

Comparison of the transcellular transport of FDG and D-glucose by the kidney epithelial cell line, LLC-PK1

This study compared the time course of transcellular transport of administered [14C] labeled FDG with that of administered GLU using the kidney epithelial cell line, LLC-PK1 to clarify the polarized distribution of trans cellular transporters.

Membrane transport mechanisms of quinidine and procainamide in renal LLC-PK1 and intestinal LS180 cells.

It is suggested that Procainamide (but not quinidine) was transported by cation transport systems in renal epithelial cells, but that both procainamide and quinazine were taken up by another cation Transport system in intestinal epithelial Cells.

Major contribution of tubular secretion to creatinine clearance in mice.

It is shown that there is a major secretory contribution to creatinine excretion mediated through the organic anion transport system and this feature adds to problems associated with measuring endogenous Creatinine filtration in mice.

Innovative strategies to improve or replace renal proximal convoluted tubule function

Promising renal replacement therapies include the development of a bioartificial kidney using functional human kidney cell models. In this study, human conditionally immortalized proximal tubular

The effect of acyclovir on the tubular secretion of creatinine in vitro

The results suggest that acyclovir does not affect the renal tubular handling of Creatinine, and hence, the pronounced, transient increase in plasma creatinine is due to decreased GFR, and not to a spurious increase in Plasma creat inine.

Renal Drug Transporters and Drug Interactions

An approach based on substrate–inhibitor associations for predicting potential tubular-based DDIs and preventing their adverse consequences is proposed, and a comprehensive list of known drug interactions with renally-expressed transporters is provided.

In vitro-in vivo extrapolation method to predict human renal clearance of drugs.

It was shown that filtration and secretion were main contributors to the renal organ clearance for all compounds, whereas reabsorption was predominant for compounds assigned to classes 1 and 2, resulting in under-predicted clearances.

The Role of Multidrug And Toxin Extrusion (MATE) in Renal Organic Cation Secretion

Whereas metformin and creatinine were shown to be competitive inhibitors of MPP, the inhibition of MATE1-mediated MPP transport produced by pyrimethamine and related analogs was not competitive but, instead, had a “linear, mixed-type” inhibitory profile suggestive of a MATE binding surface rather than a singular binding site.



Creatinine Transport by Basolateral Organic Cation Transporter hOCT2 in the Human Kidney

It is suggested that hOCT2, but not hO CT1, is responsible for the basolateral membrane transport of creatinine in the human kidney.

Kinetic Analysis of Tetraethylammonium Transport in the Kidney Epithelial Cell Line, LLC-PK1

Using a simple model, the transport of organic cation in kidney epithelial cell line, LLC-PK1, can be useful for the analysis of cation transport and drug interactions in the apical and basolateral membranes of renal tubules.

Transcellular transport of organic cation across monolayers of kidney epithelial cell line LLC-PK.

These findings suggest that these monolayers possess unidirectional transport systems for organic cations, corresponding to the secretion in the renal proximal tubules.

Cellular and molecular mechanisms of renal tubular secretion of organic anions and cations

The mechanisms mediating renal tubular secretion of organic anions and cations are described, including recent topics in this area, including the development of molecular techniques has greatly advanced the understanding of the molecular aspects of various transport processes.

H+-gradient-dependent active transport of tetraethylammonium cation in apical-membrane vesicles isolated from kidney epithelial cell line LLC-PK1.

It is suggested that apical membranes isolated from the LLC-PK1 cells retain the transport characteristics of NEt4+ similar to those observed in renal brush-border membranes.

Distinct characteristics of transcellular transport between nicotine and tetraethylammonium in LLC-PK1 cells.

It is suggested that nicotine undergoes vectorial transport from basolateral side to the apical side of LLC-PK1 monolayers in a H+ gradient-dependent manner, corresponding to the secretion in the renal tubules.

Transport of levofloxacin in a kidney epithelial cell line, LLC-PK1: interaction with organic cation transporters in apical and basolateral membranes.

The interactions of levofloxacin, a pyridonecarboxylic acid antibacterial drug, with the organic cation transport systems expressed in a pig kidney epithelial cell line, LLC-PK1, were examined and suggested that lev ofl Oxacin interacts with the apical H+/organic cation antiport system to a greater extent than with the basolateral system.

Cellular and molecular aspects of drug transport in the kidney.

Detailed information concerning molecular and cellular aspects of drug transporters expressed in the kidney has facilitated studies of the mechanisms underlying renal disposition as well as transporter-mediated drug interactions.

Extensive tubular secretion and reabsorption of creatinine in humans.

Creatinine clearance is found to be an unreliable marker for the glomerular filtration rate because of substantial tubular secretion and reabsorption of creatinine, changing in relative importance in relation to the degree of hydration.