Transcellular sodium fluxes and pump activity in Necturus gall-bladder epithelial cells.

@article{Hill1987TranscellularSF,
  title={Transcellular sodium fluxes and pump activity in Necturus gall-bladder epithelial cells.},
  author={Adrian E. Hill and Bruce S Hill},
  journal={The Journal of physiology},
  year={1987},
  volume={382},
  pages={35-49}
}
1. Transepithelial Na transport in Necturus was determined by measuring the rate of isotonic fluid flow. The rate at 20 degrees C was equivalent to 175 pmol cm-2 s-1. 2. Ouabain was effective in Necturus, binding to the Na pump in gall-bladder cells with a mean rate constant of 5.4 X 10(3) M-1 s-1. Measurement of the diffusive time constant of the free space for [3H]ouabain shows that the pump must be fully inhibited within 20 s when ouabain is applied to the serosa at 10(-3) M. 3. The serosal… CONTINUE READING