Transactivation ability of p53 transcriptional activation domain is directly related to the binding affinity to TATA-binding protein.

@article{Chang1995TransactivationAO,
  title={Transactivation ability of p53 transcriptional activation domain is directly related to the binding affinity to TATA-binding protein.},
  author={Jun Chang and Do Han Kim and Stephen Wai-Lun Lee and Kwan Yong Choi and Y C Sung},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 42},
  pages={25014-9}
}
Tumor suppressor protein p53 is a potent transcriptional activator and regulates cell growth negatively. To characterize the transcriptional activation domain (TAD) of p53, various point mutants were constructed in the context of Gal4 DNA binding domain and tested for their transactivation ability. Our results demonstrated that the positionally conserved hydrophobic residues shared with herpes simplex virus VP16 and other transactivators are essential for transactivation. Also, the negatively… CONTINUE READING

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