Trans-splicing mutants of Chlamydomonas reinhardtii

  title={Trans-splicing mutants of Chlamydomonas reinhardtii},
  author={Michel Goldschmidt-Clermont and Jacqueline Girard-Bascou and Yves Choquet and J. -D. Rochaix},
  journal={Molecular and General Genetics MGG},
In Chlamydomonas reinhardtii the three exons of the psaA gene are widely scattered on the chloroplast genome: exons 1 and 2 are in opposite orientations and distant from each other and from exon 3. The mature mRNA, encoding a core polypeptide of photosystem I, is thus probably assembled from separate precursors by splicing in trans. We have isolated and characterized a set of mutants that are deficient in the maturation of psaA mRNA. The mutants belong to 14 nuclear complementation groups and… CONTINUE READING
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