Trans-generation inheritance of methylation patterns in a tobacco transgene following a post-transcriptional silencing event.

@article{LunerovBedrichov2008TransgenerationIO,
  title={Trans-generation inheritance of methylation patterns in a tobacco transgene following a post-transcriptional silencing event.},
  author={Jana Lunerov{\'a}-Bedrichov{\'a} and Annick Bleys and Miloslava Fojtov{\'a} and Lucie Crhak Khaitova and Anna Depicker and Ale{\vs} Kovař{\'i}k},
  journal={The Plant journal : for cell and molecular biology},
  year={2008},
  volume={54 6},
  pages={1049-62}
}
We have studied the inheritance of the epigenetic state of tobacco transgenes whose expression was post-transcriptionally silenced by an invertedly repeated silencer locus. We show that, in hybrids, the coding region of the target neomycin phosphotransferase (nptII) gene was almost exclusively methylated at CG configurations, and dense non-CG methylation occurred in the 3' untranslated region. Homologous sequences in the silencer locus were heavily methylated at both CG and non-CG motifs. After… CONTINUE READING