Trans-activation of the DNA-damage signalling protein kinase Chk2 by T-loop exchange.

@article{Oliver2006TransactivationOT,
  title={Trans-activation of the DNA-damage signalling protein kinase Chk2 by T-loop exchange.},
  author={Antony W. Oliver and Angela Paul and Katherine J. Boxall and S. Elaine Barrie and G. Wynne Aherne and Michelle Dawn Garrett and Sibylle Mittnacht and Laurence H. Pearl},
  journal={The EMBO journal},
  year={2006},
  volume={25 13},
  pages={3179-90}
}
The protein kinase Chk2 (checkpoint kinase 2) is a major effector of the replication checkpoint. Chk2 activation is initiated by phosphorylation of Thr68, in the serine-glutamine/threonine-glutamine cluster domain (SCD), by ATM. The phosphorylated SCD-segment binds to the FHA domain of a second Chk2 molecule, promoting dimerisation of the protein and triggering phosphorylation of the activation segment/T-loop in the kinase domain. We have now determined the structure of the kinase domain of… CONTINUE READING