Trans-Membrane Uptake and Intracellular Metabolism of Fatty Acids in Atlantic Salmon (Salmo salar L.) Hepatocytes

@article{Zhou2010TransMembraneUA,
  title={Trans-Membrane Uptake and Intracellular Metabolism of Fatty Acids in Atlantic Salmon (Salmo salar L.) Hepatocytes},
  author={Jishu Zhou and Ingunn Stubhaug and Bente Elisabeth Torstensen},
  journal={Lipids},
  year={2010},
  volume={45},
  pages={301-311}
}
To elucidate if the trans-membrane uptake of fatty acids is protein-mediated, the uptake of oleic acid (18:1n-9), linoleic acid (18:2n-6), alpha-linolenic acid (18:3n-3), eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3) was investigated in vitro in Atlantic salmon (Salmo salar L.) primary hepatocytes. Firstly, optimal fatty acid incubation time and concentration were established for trans-membrane 18:n-9 uptake. Based on saturation kinetics, a 2-h incubation time and 37.5 μM… CONTINUE READING