Tracking chromaffin granules on their way through the actin cortex

@article{Oheim2000TrackingCG,
  title={Tracking chromaffin granules on their way through the actin cortex},
  author={Martin Oheim and Walter Stuehmer},
  journal={European Biophysics Journal},
  year={2000},
  volume={29},
  pages={67-89}
}
Quantitative time-lapse evanescent-wave imaging of individual fluorescently labelled chromaffin granules was used for kinetic analysis of granule trafficking through a ∼300-nm (1/e2) optical section beneath the plasma membrane. The mean squared displacement (MSD) was used to estimate the three-dimensional diffusion coefficient (D (3)). We calculated the granules' speed, frame-to-frame displacement and direction and their autocorrelation to identify different stages of approach to the membrane… CONTINUE READING
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