Tracking antigen-driven responses by flow cytometry: monitoring proliferation by dye dilution.

@article{Wallace2008TrackingAR,
  title={Tracking antigen-driven responses by flow cytometry: monitoring proliferation by dye dilution.},
  author={Paul K. Wallace and Joseph D. Tario and Jan L. Fisher and Stephen S Wallace and Marc S. Ernstoff and Katharine A. Muirhead},
  journal={Cytometry. Part A : the journal of the International Society for Analytical Cytology},
  year={2008},
  volume={73 11},
  pages={1019-34}
}
Cell-tracking reagents such as the green-fluorescent protein labeling dye CFSE and the red-fluorescent lipophilic membrane dye PKH26 are commonly used to monitor cell proliferation by flow cytometry in heterogeneous cell populations responding to immune stimuli. Both reagents stain cells with a bright homogeneous fluorescence, which is partitioned between daughter cells during each cell division. Because daughter cell fluorescence intensities are approximately halved after each division, the… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 40 extracted citations

Modulation of Sickle Red Blood Cell Adhesion and its Associated Changes in Biomarkers by Sulfated Nonanticoagulant Heparin Derivative.

Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis • 2016
View 9 Excerpts
Highly Influenced

Hepatocyte‐induced CD4+ T cell alloresponse is associated with major histocompatibility complex class II up‐regulation on hepatocytes and suppressible by regulatory T cells

Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society • 2018

References

Publications referenced by this paper.
Showing 1-10 of 75 references

Miniaturization and automation for measuring antigen specific T-cell responses

G Li Pira, F Ivaldi, +4 authors F. Manca
Presented at the third MASIR conference, • 2008
View 7 Excerpts
Highly Influenced

Analytical performance of a standardized single-platform MHC tetramer assay for the identification and enumeration of CMV-specific CD8+ T lymphocytes.

Cytometry. Part A : the journal of the International Society for Analytical Cytology • 2008
View 4 Excerpts
Highly Influenced

The ELISPOT assay—quality issues related to its use in a multicenter setting

D Olive, B Autran, E. Robinet
Presented at the third MASIR conference, • 2008
View 1 Excerpt

Analysis of cell differentiation by division tracking cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology • 2007

Similar Papers

Loading similar papers…