Tracking active groundwater microbes with D2O labelling to understand their ecosystem function

@article{Taubert2018TrackingAG,
  title={Tracking active groundwater microbes with D2O labelling to understand their ecosystem function},
  author={M. Taubert and S. St{\"o}ckel and P. Geesink and Sophie Girnus and N. Jehmlich and M. von Bergen and P. R{\"o}sch and J. Popp and K. K{\"u}sel},
  journal={Environmental Microbiology},
  year={2018},
  volume={20},
  pages={369–384}
}
Microbial activity is key in understanding the contribution of microbial communities to ecosystem functions. Metabolic labelling with heavy water (D2 O) leads to the formation of carbon-deuterium bonds in active microorganisms. We illustrated how D2 O labelling allows monitoring of metabolic activity combined with a functional characterization of active populations in complex microbial communities. First, we demonstrated by single cell Raman microspectroscopy that all measured bacterial cells… Expand
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