Tracking, tuning, and terminating microbial physiology using synthetic riboregulators.

  title={Tracking, tuning, and terminating microbial physiology using synthetic riboregulators.},
  author={Jarred M Callura and Daniel J. Dwyer and Farren J Isaacs and Charles R. Cantor and James J. Collins},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={107 36},
The development of biomolecular devices that interface with biological systems to reveal new insights and produce novel functions is one of the defining goals of synthetic biology. Our lab previously described a synthetic, riboregulator system that affords for modular, tunable, and tight control of gene expression in vivo. Here we highlight several experimental advantages unique to this RNA-based system, including physiologically relevant protein production, component modularity, leakage… CONTINUE READING
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