Tracer Diffusion of Proteins in DNA Solutions. 2. Green Fluorescent Protein in Crowded DNA Solutions

@inproceedings{Busch2000TracerDO,
  title={Tracer Diffusion of Proteins in DNA Solutions. 2. Green Fluorescent Protein in Crowded DNA Solutions},
  author={N. A. Busch and Thomas P. Kim and Victor A. Bloomfield},
  year={2000}
}
Proteins diffuse to their sites of action within cells in a crowded, strongly interacting environment of nucleic acids and other macromolecules. To investigate the dynamics of a typical globular protein in such an environment, we used fluorescence photobleaching recovery to measure the probe diffusion of green fluorescent protein (GFP) in dilute to highly concentrated aqueous solutions of glycerol, Ficoll 70, and persistence-length calf thymus DNA. In glycerol, GFP accurately obeyed the Stokes… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 11 CITATIONS

Dictyostelium amoebae and neutrophils can swim.

  • Proceedings of the National Academy of Sciences of the United States of America
  • 2010
VIEW 1 EXCERPT
CITES BACKGROUND