TraC of IncN plasmid pKM101 associates with membranes and extracellular high-molecular-weight structures in Escherichia coli.

@article{SchmidtEisenlohr1999TraCOI,
  title={TraC of IncN plasmid pKM101 associates with membranes and extracellular high-molecular-weight structures in Escherichia coli.},
  author={Heike Schmidt-Eisenlohr and Natalie Domke and Christian Baron},
  journal={Journal of bacteriology},
  year={1999},
  volume={181 18},
  pages={5563-71}
}
Conjugative transfer of IncN plasmid pKM101 is mediated by the TraI-TraII region-encoded transfer machinery components. Similar to the case for the related Agrobacterium tumefaciens T-complex transfer apparatus, this machinery is needed for assembly of pili to initiate cell-to-cell contact preceding DNA transfer. Biochemical and cell biological experiments presented here show extracellular localization of TraC, as suggested by extracellular complementation of TraC-deficient bacteria by helper… CONTINUE READING

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