TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR

Abstract

The advent of fluorescence-based quantitative real-time PCR (qPCR) has revolutionized the quantification of gene expression analysis in many fields, including life sciences, agriculture, forensic science, molecular diagnostics, and medicine. While SYBR Green-based qPCR is the most commonly-used platform due to its inexpensive nature and robust chemistry… (More)
DOI: 10.1371/journal.pone.0132666

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Cite this paper

@inproceedings{Zhang2015TqPCRAT, title={TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR}, author={Qian Zhang and Jing Wang and Fang Deng and Zhengjian Yan and Yinglin Rick Xia and Zhong-liang Wang and Jixing Ye and Youlin Deng and Zhonglin Zhang and Min Qiao and Ruifang Li and Sahitya K. Denduluri and Qiang Wei and Lianggong Zhao and Shun Lu and Xin Wang and Shengli Tang and Hao Liu and Hue H. Luu and Rex C. Haydon and Tong-Chuan He and Li Jiang and Guo-Chang Fan}, booktitle={PloS one}, year={2015} }