Toxicity of 5-aza-2'-deoxycytidine to mammalian cells is mediated primarily by covalent trapping of DNA methyltransferase rather than DNA demethylation.

@article{Jttermann1994ToxicityO5,
  title={Toxicity of 5-aza-2'-deoxycytidine to mammalian cells is mediated primarily by covalent trapping of DNA methyltransferase rather than DNA demethylation.},
  author={R J{\"u}ttermann and E Li and R Jaenisch},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1994},
  volume={91 25},
  pages={11797-801}
}
  • R Jüttermann, E Li, R Jaenisch
  • Published 1994 in
    Proceedings of the National Academy of Sciences…
The deoxycytidine analog 5-aza-2'-deoxycytidine (5-azadCyd) has been widely used as a DNA methylation inhibitor to experimentally induce gene expression and cellular differentiation. Prior to the availability of mutant mice with altered DNA methyltransferase levels, treatment of cells with drugs has been the only means to experimentally manipulate the level of genomic DNA methylation in mammalian cells. Substitution of DNA with 5-azadCyd leads to covalent trapping of the enzyme, thereby… CONTINUE READING
Highly Influential
This paper has highly influenced 15 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS