Toxic effects of lipid-mediated gene transfer in ventral mesencephalic explant cultures.

@article{Bauer2006ToxicEO,
  title={Toxic effects of lipid-mediated gene transfer in ventral mesencephalic explant cultures.},
  author={Matthias Bauer and Bjarne Winther Kristensen and Morten Meyer and Thomas Gasser and Hans Ruedi Widmer and Jens Zimmer and Marius Ueffing},
  journal={Basic & clinical pharmacology & toxicology},
  year={2006},
  volume={98 4},
  pages={395-400}
}
Adverse effects of cDNA and oligonucleotide delivery methods have not yet been systematically analyzed. We introduce a protocol to monitor toxic effects of two non-viral lipid-based gene delivery protocols using CNS primary tissue. Cell membrane damage was monitored by quantifying cellular uptake of propidium iodide and release of cytosolic lactate dehydrogenase to the culture medium. Using a liposomal transfection reagent, cell membrane damage was already seen 24 hr after transfection. Nestin… CONTINUE READING