Toxic effects of acetylcholinesterase on neuronal and glial-like cells in vitro

@article{Caldern1998ToxicEO,
  title={Toxic effects of acetylcholinesterase on neuronal and glial-like cells in vitro},
  author={Frances H. Calder{\'o}n and Rommy von Bernhardi and Giancarlo V. De Ferrari and Sandra Luza and Rebeca Aldunate and Nibaldo C Inestrosa},
  journal={Molecular Psychiatry},
  year={1998},
  volume={3},
  pages={247-255}
}
Acetylcholinesterase (AChE), the enzyme involved in the hydrolysis of the neurotransmitter acetylcholine, has been implicated in non-cholinergic actions which may play a role in neurodegenerative diseases such as Alzheimer's disease. To study the potential cytotoxicity of brain AChE, the effects of affinity purified AChE were analyzed on neuronal (Neuro 2a) and glial-like (B12) cells. LDH release and MTT reduction assays showed that AChE was toxic; the toxicity was dependent on the enzyme… CONTINUE READING