Toxic effects of 4‐methylthio‐3‐butenyl isothiocyanate (Raphasatin) in the rat urinary bladder without genotoxicity

  title={Toxic effects of 4‐methylthio‐3‐butenyl isothiocyanate (Raphasatin) in the rat urinary bladder without genotoxicity},
  author={Isamu Suzuki and Young-Man Cho and Tadashi Hirata and Takeshi Toyoda and Jun-ichi Akagi and Yasushi Nakamura and Azusa Sasaki and Takako Nakamura and Shigehisa Okamoto and Koji Shirota and Noboru Suetome and Akiyoshi Nishikawa and Kumiko Ogawa},
  journal={Journal of Applied Toxicology},
  pages={485 - 494}
We recently reported that 4‐methylthio‐3‐butenyl isothiocyanate (MTBITC) exerts chemopreventive effects on the rat esophageal carcinogenesis model at a low dose of 80 ppm in a diet. In contrast, some isothiocyanates (ITCs) have been reported to cause toxic effects, promotion activity, and/or carcinogenic potential in the urinary bladder of rats. In the present study, we investigated whether MTBITC had toxic effects in the urinary bladder similar to other ITCs, such as phenethyl ITC (PEITC… 

Horseradish extract promotes urinary bladder carcinogenesis when administered to F344 rats in drinking water

Although clear tumor induction was not observed, administration of relatively low‐dose HRE increased cell proliferation in the urothelium and exerted obvious promoting effects on rat urinary bladder carcinogenesis.

Effects of 3-butenyl isothiocyanate on phenotypically different prostate cancer cells.

Results show a promising role for the 3-butenyl ITC(3-BI) compound as a co-adjuvant agent in DOCE-based therapy in certain types of PC.

Subchronic toxicity evaluation of 5-hexenyl isothiocyanate, a nature identical flavoring substance from Wasabia japonica, in F344/DuCrj rats.

4-Methylthio-3-butenyl isothiocyanate (MTBITC) induced apoptotic cell death and G2/M cell cycle arrest via ROS production in human esophageal epithelial cancer cells.

Results suggest that MTBITC has chemopreventive potential for esophageal carcinogenesis by elimination of cancer cells via induction of mitochondrial apoptotic cell death, G2/M cell cycle arrest, and ROS production.

Cruciferous Vegetables, Isothiocyanates, and Bladder Cancer Prevention.

Both cell and animal studies support a potential role for isothiocyanates in bladder cancer prevention and treatment and future studies are necessary to examine clinically relevant outcomes and define guidelines on ameliorating the bladder cancer burden.

Reactivation of mutant p53 in esophageal squamous cell carcinoma by isothiocyanate inhibits tumor growth

PEITC has antitumor effects, with its ability to restore p53R248Q activity being a key molecular event responsible for these effects, and has a preferential selectivity for ESCC with p53 mutations.

Nationwide distribution of Kyo-yasai (heirloom vegetables in Kyoto) and the advantages of traditional farming methods with importance of ‘Syun’: a case of mizuna

Introduction This study aimed to review the farming activity and the administrative measure to successfully increase the distribution of heirloom vegetable “mizuna” nationwide by examining the


Involvement of Toxicity as an Early Event in Urinary Bladder Carcinogenesis Induced by Phenethyl Isothiocyanate, Benzyl Isothiocyanate, and Analogues in F344 Rats

Investigation of early changes in rat urinary bladder epithelium induced by PEITC and BITC suggests that continuous urinary epithelial cell proliferation due to cytotoxicity may play an important role in the early stage ofRat urinary bladder carcinogenesis due to oral administration of ITCs.

Strong promoting activity of phenylethyl isothiocyanate and benzyl isothiocyanate on urinary bladder carcinogenesis in F344 male rats

PEITC and BITC were shown to be strong promoters of urinary bladder carcinogenesis with some complete carcinogenic potential and tumors and PN hyperplasias in the groups treated with PEITC or BITC are characterized by downward growth.

4-Methylthio-3-butenyl isothiocyanate (raphasatin) exerts chemopreventive effects against esophageal carcinogenesis in rats

It is indicated that 4-methylthio-3-butenyl isothiocyanate may exert chemopreventive effects against N-nitrosomethylbenzylamine-induced esophageal carcinogenesis in rats and induced apoptosis, suppressed cell proliferation, and increased p21 expression when administered in the promotion phase.

Effects of dietary phenethyl isothiocyanate, ellagic acid, sulindac and calcium on the induction and progression of N-nitrosomethylbenzylamine-induced esophageal carcinogenesis in rats.

Together these findings indicate that PEITC selectively inhibits the induction but not the subsequent progression of NMBA-induced esophageal tumors; EA may repress esophagal tumor development when administered following NMBA treatment; and at the doses administered, neither sulindac nor SDC possess significant inhibitory activity against NMBA -induced esophileal carcinogenesis in the rat.

Chemopreventive effects of 4-methylthio-3-butenyl Isothiocyanate (Raphasatin) but not curcumin against pancreatic carcinogenesis in hamsters.

It is indicated that the naturally occurring isothiocyanate MTBITC may exert preventive effects against BOP-initiation of hamster pancreatic carcinogenesis and the incidence of combined pancreatic lesions was significantly decreased.

Dose-Dependent Promotion by Phenylethyl Isothiocyanate, a Known Chemopreventer, of Two-Stage Rat Urinary Bladder and Liver Carcinogenesis

It is concluded that >0.01% PEITC enhances rat urinary bladder carcinogenesis, while weakly promoting hepatocarcinogenesis, and it is suggested that <0.05%PEITC has tumorigenic potential.

Effects of seven chemicals on DNA damage in the rat urinary bladder: a comet assay study.

Chemoprevention of colonic aberrant crypt foci in Fischer rats by sulforaphane and phenethyl isothiocyanate.

Findings provide important laboratory evidence for a potential role of SFN and PEITC in the protection against colon cancer.

Genotoxic effects of allyl isothiocyanate (AITC) and phenethyl isothiocyanate (PEITC).