Toxic Trypsin Digest Fragment from the Bacillus thuringiensis Parasporal Protein.

@article{Aronson1987ToxicTD,
  title={Toxic Trypsin Digest Fragment from the Bacillus thuringiensis Parasporal Protein.},
  author={Joshua Aronson and H C Arvidson},
  journal={Applied and environmental microbiology},
  year={1987},
  volume={53 2},
  pages={416-21}
}
Enzymatic digestion in vitro of the Bacillus thuringiensis protoxin presumably releases and activates the toxin in a manner analogous to that which occurs when a B. thuringiensis sporulated fermentation preparation passes through the midgut of a lepidopteran larva. Therefore, a sporulated culture of B. thuringiensis subsp. kurstaki (serotype 3a3b) HD-263 was treated with trypsin to release an activated toxin soluble in bicarbonate buffer. A 63-kilodalton protein, toxic to cabbage looper larvae… CONTINUE READING