Towards the development of a simplified long-term organ culture method for human scalp skin and its appendages under serum-free conditions.

Abstract

Organ culture of human scalp skin is usually performed with serum-containing medium, which limits its analytical usefulness. Here we report that intact human scalp skin can be grown at the air/liquid interface in supplemented, serum-free William's E medium for more than 2 weeks. Active hair shaft growth was visible until day 16 and was significantly… (More)

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