Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces

@article{Imbeaud2005TowardsSO,
  title={Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces},
  author={Sandrine Imbeaud and Esther Graudens and Virginie Boulanger and Xavier Barlet and Patrick Zaborski and Eric Eveno and Odilo M. Mueller and Andreas Schroeder and Charles Auffray},
  journal={Nucleic Acids Research},
  year={2005},
  volume={33},
  pages={e56 - e56}
}
While it is universally accepted that intact RNA constitutes the best representation of the steady-state of transcription, there is no gold standard to define RNA quality prior to gene expression analysis. In this report, we evaluated the reliability of conventional methods for RNA quality assessment including UV spectroscopy and 28S:18S area ratios, and demonstrated their inconsistency. We then used two new freely available classifiers, the Degradometer and RIN systems, to produce user… CONTINUE READING