Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development.

@article{Shen2009TowardsCO,
  title={Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development.},
  author={Hongyuan Shen and Shou Guo Fang and Bo Chen and Guang Chen and Felicia Pei Ling Tay and Ding Xiang Liu},
  journal={Journal of virological methods},
  year={2009},
  volume={160 1-2},
  pages={
          48-56
        }
}
Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, the firefly luciferase gene and several host and viral genes (eIF3f, SARS ORF6, Dengue virus 1 core protein gene) were inserted into various positions of the IBV genome, and the… CONTINUE READING