Towards an optimized culture medium for the generation of mouse induced pluripotent stem cells.

@article{Chen2010TowardsAO,
  title={Towards an optimized culture medium for the generation of mouse induced pluripotent stem cells.},
  author={Jiekai Chen and Jing Liu and Qingkai Han and Dajiang Qin and Jianyong Xu and You Hua Chen and Jiaqi Yang and Hong Mei Song and Dongshan Yang and Meixiu Peng and Wenzhi He and Ronghui Li and Hao Wang and Yi Gan and Ke Ding and Lingwen Zeng and Liangxue Lai and Miguel A Esteban and Duanqing Pei},
  journal={The Journal of biological chemistry},
  year={2010},
  volume={285 40},
  pages={31066-72}
}
Generation of induced pluripotent stem cells from somatic cells using defined factors has potential relevant applications in regenerative medicine and biology. However, this promising technology remains inefficient and time consuming. We have devised a serum free culture medium termed iSF1 that facilitates the generation of mouse induced pluripotent stem cells. This optimization of the culture medium is sensitive to the presence of Myc in the reprogramming factors. Moreover, we could reprogram… CONTINUE READING