Towards absolute quantification of therapeutic monoclonal antibody in serum by LC-MS/MS using isotope-labeled antibody standard and protein cleavage isotope dilution mass spectrometry.

@article{Heudi2008TowardsAQ,
  title={Towards absolute quantification of therapeutic monoclonal antibody in serum by LC-MS/MS using isotope-labeled antibody standard and protein cleavage isotope dilution mass spectrometry.},
  author={Olivier Heudi and Samuel Barteau and Dieter Zimmer and J{\"o}rg Schmidt and Kurt Bill and Natalie Lehmann and Christian Bauer and Olivier Kretz},
  journal={Analytical chemistry},
  year={2008},
  volume={80 11},
  pages={4200-7}
}
Although LC-MS methods are increasingly used for the absolute quantification of proteins, the lack of appropriate internal standard (IS) hinders the development of rapid and standardized analytical methods for both in vitro and in vivo studies. Here, we have developed a novel method for the absolute quantification of a therapeutic protein, which is monoclonal antibody (mAb). The method combines liquid chromatography tandem mass spectrometry (LC-MS/MS) and protein cleavage isotope dilution mass… CONTINUE READING

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