Towards a structural biology of bacterial conjugation

@article{Silverman1997TowardsAS,
  title={Towards a structural biology of bacterial conjugation},
  author={Philip M. Silverman},
  journal={Molecular Microbiology},
  year={1997},
  volume={23}
}
  • P. Silverman
  • Published 1997
  • Biology, Medicine
  • Molecular Microbiology
Horizontal DNA transfer among bacteria (conjugation) requires the formation of secure intercellular contacts before DNA transfer can occur. The formation of such contacts among Gram‐negative bacteria is mediated by conjugative pili. Like other pili, conjugative pili are filaments extending from the surface of donor cells. They are composed, in so far as is known, entirely of conjugative pilin subunits. Here, we review the structure of F‐pilin, the subunit of which F‐pili are composed. We… Expand
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References

SHOWING 1-10 OF 31 REFERENCES
Conjugational junctions: morphology of specific contacts in conjugating Escherichia coli bacteria.
TLDR
F-plasmid-mediated bacterial conjugation was studied with hfr (traDts) and tra I mutant Escherichia coli donor strains and no specific substructure such as plasma bridges by fusion could be detected during transfer of DNA. Expand
DNA processing reactions in bacterial conjugation.
TLDR
The initiation complex for conjugative transfer of transmissible plasmids--the relaxosome--is a specific DNA-protein structure that has been isolated from cells and reconstitute from purified components in vitro. Expand
Structure and function of conjugative pili: monoclonal antibodies as probes for structural variants of F pili
TLDR
It is shown that pRS31 contains as yet uncharacterized genes required for elaboration of structurally normal F pili and that the F plasmid must contain additional genes that affect pilus structure and function. Expand
Pilus Assembly by Agrobacterium T-DNA Transfer Genes
TLDR
A genetic analysis demonstrated that virA, virG, virB1 through virB11, and virD4 are the only Ti plasmid genes necessary for pilus assembly, consistent with the view that Agrobacterium pili are required for transfer of DNA to plant cells in a process similar to that of conjugation. Expand
Analysis of the sequence and gene products of the transfer region of the F sex factor.
TLDR
This review will concentrate on the genes required for bacterial conjugation that are encoded within the transfer region (or regions) of conjugative plasmids, which has been the paradigm for conjugations since it was discovered nearly 50 years ago. Expand
The role of the pilus in recipient cell recognition during bacterial conjugation mediated by F‐like plasmids
TLDR
The results suggested that the differences in the pilin sequences were not responsible for recognizing specific groups in the LPS, OmpA or the TraT surface exclusion protein. Expand
Structure and function of conjugative pili: inducible synthesis of functional F pili by Escherichia coli K-12 containing a lac-tra operon fusion.
In vivo and in vitro recombination methods were used to construct the recombinant plasmid pTG801, in which the F-plasmid DNA transfer (tra) genes required for the formation of functional F pili wereExpand
The physiology and biochemistry of pili.
TLDR
The chapter discusses the classification scheme for pili based on morphology, function, and biochemical properties and describes three major groups of pilus—namely, conjugative, adhesive, and N-methylphenylalanine (NMePhe) pili. Expand
Comparative Biochemical Studies on F and EDP208 Conjugative Pili
TLDR
F and EDP208 pili are closely related structures and the tryptic peptide maps of the two proteins contained seven peptides with similar mobilities, suggesting considerable homology in their amino acid sequences. Expand
Bacterial conjugative plasmids mobilize DNA transfer between bacteria and yeast
TLDR
Conjugative plasmids of Escherichia coli can mobilize DNA transmission from this bacterium to the yeast Saccharomyces cerevisiae and could be evolutionary significant in promoting trans-kingdom genetic exchange. Expand
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