Towards a new reference test for surra in camels.

  title={Towards a new reference test for surra in camels.},
  author={Thao Quoc Tran and Filip Claes and Didier Verloo and Henri de Greve and Philippe B{\"u}scher},
  journal={Clinical and vaccine immunology : CVI},
  volume={16 7},
Current serological diagnosis of Trypanosoma evansi infection in camels is based on the native variable antigen type RoTat 1.2. The goal of this study was to develop a novel serological diagnostic test based on a nonvariable protein and freed from the use of rats or mice for its production. An enzyme-linked immunosorbent assay using a recombinant extracellular domain of invariant surface glycoprotein 75 (ELISA/rISG75) was developed and tested on a collection of 184 camel sera. The results were… CONTINUE READING