Toward understanding the mutagenicity of an environmental carcinogen: structural insights into nucleotide incorporation preferences.

@article{Perlow2002TowardUT,
  title={Toward understanding the mutagenicity of an environmental carcinogen: structural insights into nucleotide incorporation preferences.},
  author={Rebecca A Perlow and Suse Broyde},
  journal={Journal of molecular biology},
  year={2002},
  volume={322 2},
  pages={291-309}
}
Bulky carcinogen-DNA adducts, including (+)-trans-anti-[BP]-N(2)-dG derived from the reaction of (+)-anti-benzo[a]pyrene diol epoxide with guanine, often block the progression of DNA polymerases. However, when rare bypass of the lesions does occur, they may be misreplicated. Experimental results have shown that nucleotides are inserted opposite the (+)-trans-anti-[BP]-N(2)-dG adduct by bacteriophage T7 DNA polymerase with the order of preference A>T>or=G>C. To gain structural insights into the… CONTINUE READING