Toward rational design of protein detergent complexes: determinants of mixed micelles that are critical for the in vitro stabilization of a G-protein coupled receptor.

@article{OMalley2011TowardRD,
  title={Toward rational design of protein detergent complexes: determinants of mixed micelles that are critical for the in vitro stabilization of a G-protein coupled receptor.},
  author={Michelle A O'Malley and Matthew E Helgeson and Norman J. Wagner and Anne S. Robinson},
  journal={Biophysical journal},
  year={2011},
  volume={101 8},
  pages={1938-48}
}
Although reconstitution of membrane proteins within protein detergent complexes is often used to enable their structural or biophysical characterization, it is unclear how one should rationally choose the appropriate micellar environment to preserve native protein folding. Here, we investigated model mixed micelles consisting of a nonionic glucosylated alkane surfactant from the maltoside and thiomaltoside families, bile salt surfactant, and the steryl derivative cholesteryl hemisuccinate. We… CONTINUE READING

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