Toward quantitative fluorescence measurements with multicolor flow cytometry.

@article{Wang2008TowardQF,
  title={Toward quantitative fluorescence measurements with multicolor flow cytometry.},
  author={Lili Wang and Adolfas K. Gaigalas and Gerald E. Marti and Fatima Abbasi and Robert A. Hoffman},
  journal={Cytometry. Part A : the journal of the International Society for Analytical Cytology},
  year={2008},
  volume={73 4},
  pages={279-88}
}
A procedure is presented for calibrating the output of a multicolor flow cytometer in units of antibodies bound per cell (ABC). The procedure involves two steps. First, each of the fluorescence channels of the flow cytometer is calibrated using Ultra Rainbow beads with assigned values of equivalent number of reference fluorophores (ERF). The objective of this step is to establish a linear relation between the fluorescence signal in a given fluorescence channel of multicolor flow cytometers and… CONTINUE READING

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