Toward automated analysis of biofilm architecture: bias caused by extraneous confocal laser scanning microscopy images.

@article{Merod2007TowardAA,
  title={Toward automated analysis of biofilm architecture: bias caused by extraneous confocal laser scanning microscopy images.},
  author={Robin T Merod and Jennifer E Warren and Hope McCaslin and Stefan Wuertz},
  journal={Applied and environmental microbiology},
  year={2007},
  volume={73 15},
  pages={4922-30}
}
An increasing number of studies utilize confocal laser scanning microscopy (CLSM) for in situ visualization of biofilms and rely on the use of image analysis programs to extract quantitative descriptors of architecture. Recently, designed programs have begun incorporating procedures to automatically determine threshold values for three-dimensional CLSM image stacks. We have found that the automated threshold calculation is biased when a stack contains images lacking pixels of biological… CONTINUE READING

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