Toward an understanding of the formylation of initiator tRNA methionine in prokaryotic protein synthesis. II. A two-state model for the 70S ribosome.

Abstract

The 70S ribosomes can select the proper initiator tRNA between Met-tRNAfMet and fMet-tRNAfMet. Experiments on binding and on formation of aminoacylpuromycin, as a function of magnesium, potassium, or initiation factors, suggest a two-state equilibrium for 70S particles, involving a minor, active conformation and a major one which is not readily active. The formyl group would act as a specific trigger to select the active conformation. Experimental results are interpreted following this simple model and equilibrium parameters, together with kinetic constants of the peptidyltransferase activity, are presented.

Cite this paper

@article{Petersen1976TowardAU, title={Toward an understanding of the formylation of initiator tRNA methionine in prokaryotic protein synthesis. II. A two-state model for the 70S ribosome.}, author={Heidi Petersen and Antoine Danchin and Marianne Grunberg-Manago}, journal={Biochemistry}, year={1976}, volume={15 7}, pages={1362-9} }