Corpus ID: 212560339

Toward an Endothelial-Cell Covered Mechanical Valve ; Surface Re-Engineering and Bioreactor Testing of Mechanical Heart Valves

@inproceedings{Sierad2014TowardAE,
  title={Toward an Endothelial-Cell Covered Mechanical Valve ; Surface Re-Engineering and Bioreactor Testing of Mechanical Heart Valves},
  author={Leslie Neil Sierad and Eliza Laine Shaw and Ryan D. Launius and Shannon McBride and Cassie Storholt and Ryan Poole and Daniel Spence and Katie Miller and Lauren Sosdian and Kaity Allen and Lauren Burton and Anita Iari and Jiro Nagatomi and Dan Simionescu},
  year={2014}
}
Mechanical valve replacements for diseased heart valves have excellent long-term durability, but necessitate a lifetime anticoagulation regimen. We aimed to create a more hemocompatible device by modifying the leaflet surfaces to promote adherence and retention of endothelial cells under physiological shear forces. St. Jude Medical mechanical valves were autoclaved, plasma treated, coated with a collagen/fibronectin solution, and seeded with porcine aortic endothelial cells. The next day, the… Expand

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References

SHOWING 1-10 OF 30 REFERENCES
Design and Testing of a Pulsatile Conditioning System for Dynamic Endothelialization of Polyphenol-Stabilized Tissue Engineered Heart Valves
TLDR
This work hypothesized that reversibly stabilized acellular porcine valves, seeded with endothelial cells and conditioned in pulsatile bioreactors would pave the way for next generations of tissue engineered heart valves (TEHVs). Expand
Assembly and testing of stem cell-seeded layered collagen constructs for heart valve tissue engineering.
TLDR
A novel approach to heart valve tissue engineering based on bioreactor conditioning of mesenchymal stem cell-seeded, valve-shaped constructs assembled from layered collagenous scaffolds is proposed, indicating feasibility of this approach. Expand
Endothelialization of mechanical heart valves in vitro with cultured adult human cells.
TLDR
This study demonstrates that mechanical heart valves do not contain cytotoxic compounds and demonstrates that cultured adult human endothelial cells are able to form a monolayer when seeded on monostrut and bileaflet valve prostheses. Expand
Experimental in vitro endothelialization of cardiac valve leaflets.
This study reports our results with vitro endothelialization of fresh nonpreserved homograft valve leaflets compared with mild alternatively preserved valves and valves treated by preservationExpand
Attachment, morphology and adherence of human endothelial cells to vascular prosthesis materials under the action of shear stress.
TLDR
The degree to which human umbilical venous endothelial cells adhered to three materials commonly used polymeric vascular prosthesis that had been coated with the same commercial extra cellular matrix proteins, and after exposure to fluid shear stresses representative of femoro-distal bypass in a cone-and-plate shearing device was examined. Expand
Complete dynamic repopulation of decellularized heart valves by application of defined physical signals-an in vitro study.
TLDR
This study demonstrated the superiority of the hydrodynamic approach of cellular reseeding to replace decellularized porcine heart valves with ovine cells with almost complete preservation of extracellular matrix integrity. Expand
Determining cell seeding dosages for tissue engineering human pulmonary valves.
TLDR
Different seeding modes suggest a tradeoff between surface proliferation resulting in higher absolute cell numbers for static seeding versus fewer cells in a cyclic pressure bioreactor but with a greater percentage having migrated into the matrix. Expand
Tissue Engineering of Human Heart Valve Leaflets: A Novel Bioreactor for a Strain-Based Conditioning Approach
TLDR
The strain-based conditioning approach, using both prestrain and dynamic strains, offers new possibilities for bioreactor design and optimization of tissue properties towards a tissue-engineered aortic human heart valve replacement. Expand
Pathology of Substitute Heart Valves: New Concepts and Developments
TLDR
All types of contemporary cardiac valve substitutes suffer deficiencies and complications that limit their success, and the most promising anticalcification techniques are preimplantation treatments of glutaraldehyde fixed tissue with either detergents such as sodium dodecyl sulfate, 2‐amino oleic acid, cations such as Fe3+ or Al3+, or tissue preservation alternatives to aldehydes, such as ethane hydroxydiphosphonate. Expand
Improved endothelial cell seeding with cultured cells and fibronectin-coated grafts.
TLDR
Seeding efficiency is significantly better in all groups of coated grafts compared to uncoated grafts and fibronectin coating increased mean platelet accumulation on the entire graft luminal surface, but not to a statistically significant degree. Expand
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