Toward a code for the interactions of zinc fingers with DNA: selection of randomized fingers displayed on phage.

@article{Choo1994TowardAC,
  title={Toward a code for the interactions of zinc fingers with DNA: selection of randomized fingers displayed on phage.},
  author={Y. E. Choo and Aaron Klug},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1994},
  volume={91 23},
  pages={11163-7}
}
We have used two selection techniques to study sequence-specific DNA recognition by the zinc finger, a small, modular DNA-binding minidomain. We have chosen zinc fingers because they bind as independent modules and so can be linked together in a peptide designed to bind a predetermined DNA site. In this paper, we describe how a library of zinc fingers displayed on the surface of bacteriophage enables selection of fingers capable of binding to given DNA triplets. The amino acid sequences of… CONTINUE READING