Toward a clinical molecular scanner for proteome research: parallel protein chemical processing before and during western blot.

@article{Bienvenut1999TowardAC,
  title={Toward a clinical molecular scanner for proteome research: parallel protein chemical processing before and during western blot.},
  author={Willy Vincent Bienvenut and Jean Charles S{\'a}nchez and Abderrahim Karmime and V Rouge and K. -G. Rose and P A Binz and Denis F. Hochstrasser},
  journal={Analytical chemistry},
  year={1999},
  volume={71 21},
  pages={4800-7}
}
To increase the throughput of protein identification and characterization in proteome studies, we investigated three methods of performing protein digestion in parallel. The first, which we term "one-step digestion-transfer" (OSDT), is based on protein digestion during the transblotting process. It involves the use of membranes containing immobilized trypsin which are intercalated between the gel and a PVDF collecting membrane. During electrotransfer, some digestion of the transferred proteins… CONTINUE READING