Toward Système International d'Unité-traceable protein quantification: from amino acids to proteins.

  title={Toward Syst{\`e}me International d'Unit{\'e}-traceable protein quantification: from amino acids to proteins.},
  author={William I Burkitt and Caroline Pritchard and Cristian Arsene and Andr{\'e} Henrion and David M. Bunk and Gavin O'Connor},
  journal={Analytical biochemistry},
  volume={376 2},
Here we present a demonstration of the proof of principle that absolute concentration of a protein within a mixture of other proteins can be measured with SI traceability. The method used was based on tryptic digestion of a protein followed by quantification using double exact matching isotope dilution mass spectrometry (IDMS) of the peptides released. To provide full SI traceability to measurements of protein concentration we demonstrated a method of SI traceable peptide quantification in… CONTINUE READING