Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions

  title={Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions},
  author={Sophie M Morgani and Maurice A. Canham and Jennifer Nichols and Alexei A. Sharov and Rosa Portero Migueles and Minoru S. H. Ko and Joshua M Brickman},
  booktitle={Cell reports},
Embryonic stem cells (ESCs) are derived from mammalian embryos during the transition from totipotency, when individual blastomeres can make all lineages, to pluripotency, when they are competent to make only embryonic lineages. ESCs maintained with inhibitors of MEK and GSK3 (2i) are thought to represent an embryonically restricted ground state. However, we observed heterogeneous expression of the extraembryonic endoderm marker Hex in 2i-cultured embryos, suggesting that 2i blocked development… CONTINUE READING
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