Total synthesis of a human leukocyte interferon gene

@article{Edge1981TotalSO,
  title={Total synthesis of a human leukocyte interferon gene},
  author={Michael D. Edge and April R. Greene and G. R. Heathcliffe and Peter A. Meacock and Wolfgang Schuch and Denis B. Scanlon and T. C. Atkinson and C. R. Newton and Alexander F. Markham},
  journal={Nature},
  year={1981},
  volume={292},
  pages={756-762}
}
A 514-base pair fragment of double-stranded DNA coding for human interferon-α1 (166 amino acid residues), and containing initiation and termination signals plus appropriate restriction enzyme sites for plasmid insertion, has been totally synthesized. The synthesis involved preparation of 66 oligodeoxyribonucleotides, ranging in size from 14 to 21 residues, plus 1 deoxydecanucleotide, by rapid, solid phase procedures, and enzymatic ligation of the oligonucleotides. After ligation of the… Expand
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References

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Synthesis in E. coli of a polypeptide with human leukocyte interferon activity
Double-stranded cDNA prepared from the 12S fraction of poly (A) RNA from interferon (IF)-producing human leukocytes was cloned in Escherichia coli using the pBR322 vector. One of the resulting clonesExpand
Isolation and structure of a human fibroblast interferon gene
TLDR
From the nucleotide sequence of the gene, the complete amino acid sequence of human fibroblast interferon was deduced and the protein is 166 amino acids long and is preceded by a 21-amino acid signal sequence. Expand
Human leukocyte interferon produced by E. coli is biologically active
TLDR
A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli, and protected squirrel monkeys from lethal encephalomyocarditis virus infection. Expand
The nucleotide sequence of human fibroblast interferon cDNA.
TLDR
The data reported predict the hitherto unknown amino acid sequence of human fibroblast interferon and its putative signal peptide. Expand
Expression of human fibroblast interferon gene in Escherichia coli
TLDR
Antiviral activity, whose physico-chemical, immunological and biological characteristics closely corresponded to those of authentic human fibroblast Interferon, was synthesized and processed to a size compatible with mature but unglycosylated authentic product. Expand
The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcriptase
TLDR
The complete amino acid sequence of mature human fibroblast interferon, a polypeptide of 166 amino acids, is determined by repeating this process with one more primer. Expand
The nucleotide sequence of a cloned human leukocyte interferon cDNA.
TLDR
The nucleotide sequence of the human leukocyte interferon cDNA carried in hybrid plasmid Z-pBR322(Pst)/HcIF-2h, which has been shown to direct the formation of a polypeptide with human leucocyteinterferon activity, suggests that these two interferons are encoded by two non-allelic genes. Expand
The amino-terminal sequence of human fibroblast interferon as deduced from reverse transcripts obtained using synthetic oligonucleotide primers.
TLDR
An oligonucleotide capable of priming specifically the reverse transcription of human fibroblast interferon mRNA present within a total mRNA population is predicted and synthesised. Expand
Production of biologically active N alpha-desacetylthymosin alpha 1 in Escherichia coli through expression of a chemically synthesized gene.
TLDR
Results of a guinea pig migration inhibition factor assay, a terminal deoxyribonucleotidyl transferase (TdT) assay, and radioimmunoassay indicate that the N alpha-desacetylthymosin alpha 1 produced by deoxy ribonucleic acid (DNA) cloning techniques has biological activity equivalent to that of the native hormone. Expand
Bacterial synthesis of a novel human leukocyte interferon.
TLDR
A novel human leukocyte interferon cDNA clone (LeIF B) was identified in a cDNA library prepared using polyadenylated mRNA of a myeloblastoid cell line using an expression plasmid which directs the synthesis in E. coli of 8 x 10(7) Interferon units per liter of culture. Expand
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