Total synthesis of Escherichia coli with a recoded genome

  title={Total synthesis of Escherichia coli with a recoded genome},
  author={Julius Fredens and Kaihang Wang and Daniel de la Torre and Louise F. H. Funke and Wesley E. Robertson and Yonka Christova and Tiongsun Chia and Wolfgang Hellmut Schmied and Daniel L. Dunkelmann and V{\'a}clav Ber{\'a}nek and Chayasith Uttamapinant and Andres Gonzalez Llamazares and Thomas S. Elliott and Jason W. Chin},
Nature uses 64 codons to encode the synthesis of proteins from the genome, and chooses 1 sense codon—out of up to 6 synonyms—to encode each amino acid. [] Key Method We create a variant of Escherichia coli with a four-megabase synthetic genome through a high-fidelity convergent total synthesis. Our synthetic genome implements a defined recoding and refactoring scheme—with simple corrections at just seven positions—to replace every known occurrence of two sense codons and a stop codon in the genome. Thus, we…

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Codon Effect on the Entire Genome Based upon Genome-Wide Recoded Escherichia coli

  • Yi Huang
  • Biology
    2021 IEEE 9th International Conference on Bioinformatics and Computational Biology (ICBCB)
  • 2021
It was observed that expression levels of the genes associated with cell membrane almost increase, while those related to protein production nearly decrease, and the overall expression of TCA cycle goes up, while glycolysis holds most genes with lessened expressions.

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