• DOI:10.1038/s41586-019-1192-5
Corpus ID: 205571025

Total synthesis of Escherichia coli with a recoded genome

@article{Fredens2019TotalSO,
  title={Total synthesis of Escherichia coli with a recoded genome},
  author={Julius Fredens and K. Wang and D. Torre and Louise F. H. Funke and Wesley E. Robertson and Y. Christova and Tiongsun Chia and W. Schmied and Daniel L. Dunkelmann and V. Ber{\'a}nek and Chayasith Uttamapinant and A. G. Llamazares and Thomas S. Elliott and J. Chin},
  journal={Nature},
  year={2019},
  volume={569},
  pages={514-518}
}

• Julius Fredens, K. Wang, +11 authors J. Chin
• Published 2019
• Medicine, Biology
• Nature

Nature uses 64 codons to encode the synthesis of proteins from the genome, and chooses 1 sense codon—out of up to 6 synonyms—to encode each amino acid. [...] Key Method We create a variant of Escherichia coli with a four-megabase synthetic genome through a high-fidelity convergent total synthesis. Our synthetic genome implements a defined recoding and refactoring scheme—with simple corrections at just seven positions—to replace every known occurrence of two sense codons and a stop codon in the genome. Thus, we…Expand

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