Total synthesis and functional properties of the membrane-intrinsic protein phospholamban.

@article{Vorherr1993TotalSA,
  title={Total synthesis and functional properties of the membrane-intrinsic protein phospholamban.},
  author={T E Vorherr and Antoni Wrzosek and Michele Chiesi and Ernesto Carafoli},
  journal={Protein science : a publication of the Protein Society},
  year={1993},
  volume={2 3},
  pages={339-47}
}
The membrane-intrinsic protein phospholamban (PLN), the regulatory protein of the sarcoplasmic reticulum (SR) Ca(2+)-ATPase, was chemically synthesized. The synthesis was accomplished by double couplings and efficient capping procedures, thus eliminating hydrophobic failure sequences. The crude peptide was purified by high-performance liquid chromatographic ion exchange and gel permeation chromatography in chloroform-methanol mixtures. Ion spray mass spectroscopy showed that the product had the… CONTINUE READING

Similar Papers

Loading similar papers…