Total synthesis and cloning of a gene coding for the ribonuclease S protein.

@article{Nambiar1984TotalSA,
  title={Total synthesis and cloning of a gene coding for the ribonuclease S protein.},
  author={Krishnan P. Nambiar and Joseph Stackhouse and Dietrich Stauffer and Woodward - Clyde Consultants R. P. Kennedy and Jon Eldredge and S. A. Benner},
  journal={Science},
  year={1984},
  volume={223 4642},
  pages={1299-301}
}
A gene for ribonuclease S protein, has been chemically synthesized and cloned. The gene is designed to have 25 specific restriction endonuclease sites spaced at short intervals, permitting its structure to be rapidly modified. This flexibility facilitates tests of hypotheses relating the primary structure of the enzyme to its physical and catalytic behavior. 

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