Total internal reflection fluorescence microscopy: application to substrate-supported planar membranes

@article{Thomson1993TotalIR,
  title={Total internal reflection fluorescence microscopy: application to substrate-supported planar membranes},
  author={Nancy L. Thomson and Kenneth H. Pearce and Helen V. Hsieh},
  journal={European Biophysics Journal},
  year={1993},
  volume={22},
  pages={367-378}
}
The use of total internal reflection illumination in fluorescence microscopy (TIRFM) is reviewed with emphasis on application to fluorescent macromolecules that specifically and reversibly bind to planar model membranes supported on glass or quartz substrates. Several methods for characterizing macromolecular motion and organization are discussed: the measurement of equilibrium binding curves to obtain values for equilibrium binding constants; the measurement of fluorescence photobleaching… CONTINUE READING

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