Total internal reflection fluorescence correlation spectroscopy for counting molecules at solid/liquid interfaces.

@article{Hansen1998TotalIR,
  title={Total internal reflection fluorescence correlation spectroscopy for counting molecules at solid/liquid interfaces.},
  author={R L Hansen and Joel M Harris},
  journal={Analytical chemistry},
  year={1998},
  volume={70 13},
  pages={2565-75}
}
Fluorescence correlation spectroscopy, using tota internal reflection excitation (TIRFCS), is developed as a method to allow quantitative determination of molecular populations at solid/liquid interfaces. Population fluctuations of fluorescent molecules at the interface are observed as excess low-frequency noise on a fluorescence signal. Since the noise arises from molecular origins, its magnitude can be evaluated by Poisson statistics to determine the number of molecules in the interface… CONTINUE READING