Total internal reflection fluorescence: applications in cellular biophysics.

  title={Total internal reflection fluorescence: applications in cellular biophysics.},
  author={Nancy L. Thompson and B. Christoffer Lagerholm},
  journal={Current opinion in biotechnology},
  volume={8 1},
Molecular interactions occurring on or near cell membrane surfaces are expected to have different properties from those occurring in bulk solutions. One particularly useful technique for studying surface-associated processes at the molecular level is total internal reflection fluorescence. In this method, the evanescent field from an internally reflected excitation source selectively excites fluorescent molecules on or near a surface. Evanescent excitation has been used recently with a variety… CONTINUE READING

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