Total Vitamin D Assay Comparison of the Roche Diagnostics “Vitamin D Total” Electrochemiluminescence Protein Binding Assay with the Chromsystems HPLC Method in a Population with both D2 and D3 forms of Vitamin D

@inproceedings{AbdelWareth2013TotalVD,
  title={Total Vitamin D Assay Comparison of the Roche Diagnostics “Vitamin D Total” Electrochemiluminescence Protein Binding Assay with the Chromsystems HPLC Method in a Population with both D2 and D3 forms of Vitamin D},
  author={Laila Abdel-Wareth and Afrozul Haq and Andrew Turner and Shoukat Hussain Khan and Arwa Salem and Faten Mustafa and Nafiz Hussein and Fasila Pallinalakam and L Shane Grundy and Gemma Patras and Jaishen Rajah},
  booktitle={Nutrients},
  year={2013}
}
This study compared two methods of assaying the 25-hydroxylated metabolites of cholecalciferol (vitamin D3) and ergocalciferol (vitamin D2). A fully automated electrochemiluminescence assay from Roche Diagnostics and an HPLC based method from Chromsystems were used to measure vitamin D levels in surplus sera from 96 individuals, where the majority has the D2 form of the vitamin. Deming regression, concordance rate, correlation and Altman Bland agreement were performed. Seventy two subjects (75… CONTINUE READING